Recombinant Mouse Nidogen-1 (Nid1), partial | CSB-EP015802MO

Recombinant Mouse Nidogen-1 (Nid1), partial | CSB-EP015802MO | Cusabio

Alternative Name(s): Entactin

Gene Names: Nid1

Research Areas: Others

Organism: Mus musculus (Mouse)

AA Sequence: SPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQLSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDA

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 428-665aa

Sequence Info: Partial

MW: 30.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Sulfated glycoprotein widely distributed in basent mbranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-Extracellular domain matrix interactions.

Reference: Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo.Lee H.K., Seo I.A., Park H.K., Park H.T.FEBS Lett. 580:2253-2257(2006)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Involvement in disease:

Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P10493

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A