Recombinant Mouse Glutamate decarboxylase 2 (Gad2) | CSB-EP009160MO

Recombinant Mouse Glutamate decarboxylase 2 (Gad2) | CSB-EP009160MO | Cusabio

Alternative Name(s): 65KDA glutamic acid decarboxylase ;GAD-65Glutamate decarboxylase 65KDA isoform

Gene Names: Gad2

Research Areas: Others

Organism: Mus musculus (Mouse)

AA Sequence: MASPGSGFWSFGSEDGSADPENPGTARAWCQVAQKFTGGIGNKLCALLYGDSGKPAEGGGSVTSRAATGKVACTCDQKPCNCPKGDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILTHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMLIARYKMFPEVKEKGMAAVPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEVKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQSCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHIDKCLELAEYLYTIIKNREGYEMVFDGKPQHTNVCFWFVPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-585aa

Sequence Info: Full Length

MW: 69.2 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Catalyzes the production of GABA.

Reference: Mice lacking the 65KDA isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures.Asada H., Kawamura Y., Maruyama K., Kume H., Ding R.G., Ji F.Y., Kanbara N., Kuzume H., Sanbo M., Yagi T., Obata K.Biochem. Biophys. Res. Commun. 229:891-895(1996)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes the production of GABA.

Involvement in disease:

Subcellular Location: Cytoplasm, cytosol, Cytoplasmic vesicle, Cell junction, synapse, presynaptic cell membrane, Lipid-anchor, Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side

Protein Families: Group II decarboxylase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P48320

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A