Recombinant Mouse Embigin (Emb) | CSB-CF007631MO

Recombinant Mouse Embigin (Emb) | CSB-CF007631MO | Cusabio

Alternative Name(s): Teratocarcinoma glycoprotein Gp-70 (Gp70)

Gene Names: Emb

Research Areas: Tags & Cell Markers

Organism: Mus musculus (Mouse)

AA Sequence: DPTDPTFTSLPVREEMMAKYSNLSLKSCNISVTEKSNVSVEENVILEKPSHVELKCVYTATKDLNLMNVTWKKDDEPLETTGDFNTTKMGNTLTSQYRFIVFNSKQLGKYSCVFGEKELRGTFNIHVPKAHGKKKSLIAYVGDSTVLKCVCQDCLPLNWTWYMGNETAQVPIDAHSNEKYIINGSHANETRLKIKHLLEEDGGSYWCRATFQLGESEEQNELVVLSFLVPLKPFLAILAEVILLVAIILLCEVYTHKKKNDPDAGKEFEQIEQLKSDDSNGIENNVPRYRKTDSADQ

Source: in vitro E.coli expression system

Tag Info: N-terminal 10xHis-tagged

Expression Region: 34-330aa

Sequence Info: Full Length of Mature Protein

MW: 36.4 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Plays a role in targeting the monocarboxylate transporters SLC16A1 and SLC16A7 to the cell membrane. Plays a role in the outgrowth of motoneurons and in the formation of neuromuscular junctions. Following muscle denervation, promotes nerve terminal sprouting and the formation of additional acetylcholine receptor clusters at synaptic sites without affecting terminal Schwann cell number or morphology. Delays the retraction of terminal sprouts following re-innervation of denervated endplates.

Reference: "Large-scale screen for genes controlling mammalian embryogenesis, using high-throughput gene expression analysis in mouse embryos." Neidhardt L., Gasca S., Wertz K., Obermayr F., Worpenberg S., Lehrach H., Herrmann B.G. Mech. Dev. 98:77-94(2000)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Plays a role in targeting the monocarboxylate transporters SLC16A1 and SLC16A7 to the cell membrane (By similarity). Plays a role in the outgrowth of motoneurons and in the formation of neuromuscular junctions. Following muscle denervation, promotes nerve terminal sprouting and the formation of additional acetylcholine receptor clusters at synaptic sites without affecting terminal Schwann cell number or morphology. Delays the retraction of terminal sprouts following re-innervation of denervated endplates.

Involvement in disease:

Subcellular Location: Cell membrane, Single-pass type I membrane protein, Cell junction, synapse

Protein Families:

Tissue Specificity: Only member of the immunoglobulin superfamily to be expressed in embryonal carcinoma cells, which resemble multipotential cells of early embryos.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P21995

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A