Recombinant Mouse Cytochrome P450 2E (Cyp2e1) | CSB-YP006425MO

Recombinant Mouse Cytochrome P450 2E (Cyp2e1) | CSB-YP006425MO | Cusabio

Alternative Name(s): 4-nitrophenol 2-hydroxylase (EC:1.14.13.n7) CYPIIE1 Cytochrome P450-ALC Cytochrome P450-J

Gene Names: Cyp2e1

Research Areas: Others

Organism: Mus musculus (Mouse)

AA Sequence: AVLGITVALLVWIATLLLVSIWKQIYRSWNLPPGPFPIPFFGNIFQLDLKDIPKSLTKLAKRFGPVFTLHLGQRRIVVLHGYKAVKEVLLNHKNEFSGRGDIPVFQEYKNKGIIFNNGPTWKDVRRFSLSILRDWGMGKQGNEARIQREAHFLVEELKKTKGQPFDPTFLIGCAPCNVIADILFNKRFDYDDKKCLELMSLFNENFYLLSTPWIQAYNYFSDYLQYLPGSHRKVMKNVSEIRQYTLGKAKEHLKSLDINCPRDVTDCLLIEMEKEKHSQEPMYTMENISVTLADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRAPAVRDRMNMPYMDAVVHEIQRFINLVPSNLPHEATRDTVFRGYVIPKGTVVIPTLDSLLFDNYEFPDPETFKPEHFLNENGKFKYSDYFKAFSAGKRVCVGEGLARMELFLLLSAILQHFNLKSLVDPKDIDLSPVTIGFGSIPREFKLCVIPRS

Source: Yeast

Tag Info: N-terminal 6xHis-tagged

Expression Region: 2-493aa

Sequence Info: Full Length of Mature Protein

MW: 58.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites.

Reference: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project Team Genome Res. 14:2121-2127(2004)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites.

Involvement in disease:

Subcellular Location: Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane, Peripheral membrane protein

Protein Families: Cytochrome P450 family

Tissue Specificity: Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q05421

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A