Recombinant Mouse Chymotrypsin-like elastase family member 3B (Cela3b) | CSB-YP875103MO
- 3 - 7 Working Days
Recombinant Mouse Chymotrypsin-like elastase family member 3B (Cela3b) | CSB-YP875103MO | Cusabio
Alternative Name(s): Elastase IIIB Elastase-3B Protease E Ela3, Ela3b
Gene Names: Cela3b
Research Areas: Cell Biology
Organism: Mus musculus (Mouse)
AA Sequence: VVNGEEAVPHSWPWQVSLQYEKDGSFHHTCGGSLITPDWVLTAGHCISTSRTYQVVLGEHERGVEEGQEQVIPINAGDLFVHPKWNSMCVSCGNDIALVKLSRSAQLGDAVQLACLPPAGEILPNGAPCYISGWGRLSTNGPLPDKLQQALLPVVDYEHCSRWNWWGLSVKTTMVCAGGDIQSGCNGDSGGPLNCPADNGTWQVHGVTSFVSSLGCNTLRKPTVFTRVSAFIDWIEETIANN
Tag Info: N-terminal 6xHis-tagged and C-terminal Myc-tagged
Expression Region: 28-269aa
Sequence Info: Full Length of Mature Protein
MW: 29.6 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Efficient protease with alanine specificity but only little elastolytic activity.
Reference: "Osteopontin is a novel marker of pancreatic ductal tissues and of undifferentiated pancreatic precursors in mice." Kilic G., Wang J., Sosa-Pineda B. Dev. Dyn. 235:1659-1667(2006)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Efficient protease with alanine specificity but only little elastolytic activity.
Involvement in disease:
Protein Families: Peptidase S1 family, Elastase subfamily
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q9CQ52
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A