Recombinant Mouse Chymotrypsin-like elastase family member 3B (Cela3b) | CSB-EP875103MO

Recombinant Mouse Chymotrypsin-like elastase family member 3B (Cela3b) | CSB-EP875103MO | Cusabio

Alternative Name(s): Elastase IIIB Elastase-3B Protease E

Gene Names: Cela3b

Research Areas: Cell Biology

Organism: Mus musculus (Mouse)

AA Sequence: VVNGEEAVPHSWPWQVSLQYEKDGSFHHTCGGSLITPDWVLTAGHCISTSRTYQVVLGEHERGVEEGQEQVIPINAGDLFVHPKWNSMCVSCGNDIALVKLSRSAQLGDAVQLACLPPAGEILPNGAPCYISGWGRLSTNGPLPDKLQQALLPVVDYEHCSRWNWWGLSVKTTMVCAGGDIQSGCNGDSGGPLNCPADNGTWQVHGVTSFVSSLGCNTLRKPTVFTRVSAFIDWIEETIANN

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 28-269aa

Sequence Info: Full Length of Mature Protein

MW: 31.1 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Efficient protease with alanine specificity but only little elastolytic activity.

Reference: "Osteopontin is a novel marker of pancreatic ductal tissues and of undifferentiated pancreatic precursors in mice." Kilic G., Wang J., Sosa-Pineda B. Dev. Dyn. 235:1659-1667(2006)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Efficient protease with alanine specificity but only little elastolytic activity.

Involvement in disease:

Subcellular Location:

Protein Families: Peptidase S1 family, Elastase subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9CQ52

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A