Recombinant Mouse Bloom syndrome protein homolog (Blm), partial | CSB-EP002715MO
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Recombinant Mouse Bloom syndrome protein homolog (Blm), partial | CSB-EP002715MO | Cusabio
Alternative Name(s): RecQ helicase homolog
Gene Names: Blm
Research Areas: Neuroscience
Organism: Mus musculus (Mouse)
AA Sequence: INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ
Tag Info: N-terminal 10xHis-tagged and C-terminal MYC-tagged
Expression Region: 684-859aa
Sequence Info: Partial
MW: 24.7 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE)
Reference: "Point mutations causing Bloom's syndrome abolish ATPase and DNA helicase activities of the BLM protein." Bahr A., de Graeve F., Kedinger C., Chatton B. Oncogene 17:2565-2571(1998)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction
Involvement in disease:
Subcellular Location: Nucleus
Protein Families: Helicase family, RecQ subfamily
Tissue Specificity: Highly expressed in testis 12-14 days after birth (corresponding to the pachytene phase) and at much lower levels in brain, heart, liver, lung, thymus, kidney and spleen (PubMed:9840919, PubMed:27010503). Expressed in bone marrow (PubMed:27010503).
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: O88700
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A