Recombinant Mouse Bloom syndrome protein homolog (Blm), partial | CSB-EP002715MO

Recombinant Mouse Bloom syndrome protein homolog (Blm), partial | CSB-EP002715MO | Cusabio

Alternative Name(s): RecQ helicase homolog

Gene Names: Blm

Research Areas: Neuroscience

Organism: Mus musculus (Mouse)

AA Sequence: INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal MYC-tagged

Expression Region: 684-859aa

Sequence Info: Partial

MW: 24.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE)

Reference: "Point mutations causing Bloom's syndrome abolish ATPase and DNA helicase activities of the BLM protein." Bahr A., de Graeve F., Kedinger C., Chatton B. Oncogene 17:2565-2571(1998)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction

Involvement in disease:

Subcellular Location: Nucleus

Protein Families: Helicase family, RecQ subfamily

Tissue Specificity: Highly expressed in testis 12-14 days after birth (corresponding to the pachytene phase) and at much lower levels in brain, heart, liver, lung, thymus, kidney and spleen (PubMed:9840919, PubMed:27010503). Expressed in bone marrow (PubMed:27010503).

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: O88700

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A