Recombinant Mouse Alpha-defensin 1 (Defa1) | CSB-EP006652MO

Recombinant Mouse Alpha-defensin 1 (Defa1) | CSB-EP006652MO | Cusabio

Alternative Name(s): Cryptdin-1 Defensin-related cryptdin peptide

Gene Names: Defa1

Research Areas: Immunology

Organism: Mus musculus (Mouse)

AA Sequence: LRDLVCYCRSRGCKGRERMNGTCRKGHLLYTLCCR

Source: E.coli

Tag Info: N-terminal 6xHis-GST-tagged

Expression Region: 59-93aa

Sequence Info: Full Length of Mature Protein

MW: 34.1 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.

Reference: "Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium." Ouellette A.J., Greco R.M., James M., Frederick D., Naftilan J., Fallon J.T. J. Cell Biol. 108:1687-1695(1989)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Alpha-defensin family

Tissue Specificity: Paneth cells of the small bowel.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P11477

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A