Recombinant Mouse Adipocyte enhancer-binding protein 1 (Aebp1), partial | CSB-EP723715MO

Recombinant Mouse Adipocyte enhancer-binding protein 1 (Aebp1), partial | CSB-EP723715MO | Cusabio

Alternative Name(s): Aortic carboxypeptidase-like protein

Gene Names: Aebp1

Research Areas: Epigenetics and Nuclear Signaling

Organism: Mus musculus (Mouse)

AA Sequence: QTVLTDDEIEEFLEGFLSELETQSPPREDDVEVQPLPEPTQRPRKSKAGGKQRADVEVPPEKNKDKEKKGKKDKGPKATKPLEGSTRPTKKPKEKPPKATKKPKEKPPKATKKPKEKPPKATKKPKEKPPKATKRPSAGKKFSTVAPLETLDRLLPSPSNPSAQELPQKRDTPFPNAWQGQGEETQVEAKQPRPEPEEETEMPTLDYNDQIEKEDYEDFEYIRRQKQPRPTPSRRRLWPERPEEKTEEPEERKEVEPPLKPLLPPDYGDSYVIPNYDDLDYYFPHPPPQKPDVGQEVDEEKEEMKKPKKEGSSPKEDTEDKWTVEKNKDHKGPRKGEELEEEWAP

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 26-370aa

Sequence Info: Partial

MW: 55.8 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Isoform 2 may positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. Isoform 2 may also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I-kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor.

Reference: "A eukaryotic transcriptional repressor with carboxypeptidase activity." He G.-P., Muise A.M., Li A.W., Ro H.-S. Nature 378:92-96(1995)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Isoform 2 may positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. Isoform 2 may also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I-kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor.

Involvement in disease:

Subcellular Location: Isoform 1: Secreted, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm, Nucleus

Protein Families: Peptidase M14 family

Tissue Specificity: Isoform 1 and isoform 2 are expressed in adipose tissue, brain, heart, kidney, liver, lung, skeletal muscle, small intestine, spleen and testis. Isoform 2 is expressed in macrophages. Expressed in aorta, preadipocytes, adipocyte tissue, brain, heart, liver, lung, skeletal muscle, skin and spleen (at protein level).

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q640N1

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A