Recombinant Mouse 5-aminolevulinate synthase, nonspecific, mitochondrial (Alas1) | CSB-EP844984MO

Recombinant Mouse 5-aminolevulinate synthase, nonspecific, mitochondrial (Alas1) | CSB-EP844984MO | Cusabio

Alternative Name(s): 5-aminolevulinic acid synthase 1 (Delta-ALA synthase 1) (Delta-aminolevulinate synthase 1) (ALAS-H)

Gene Names: Alas1

Research Areas: Cardiovascular

Organism: Mus musculus (Mouse)

AA Sequence: QVKETPPANEKEKTAKAAVQQAPDESQMAQTPDGTQLPSGHPSPATSQGSGSKCPFLAAQLSQTGSSVFRKASLELQEDVQEMHAVRKEAAQSPVPPSLVNVKTDGEDPSRLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDHFFEKKIDEKKNDHTYRVFKTVNRRAQIFPMADDYTDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMETVKQHGAGAGGTRNISGTSKFHVELEQALADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDSGNHASMIQGIRNSRVPKYIFRHNDVNHLRELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSLLIDTVRSYAAGFIFTTSLPPMLLAGALESVRILKSSEGRALRRQHQRNVKLLRQMLMDAGLPVIHCPSHIIPVRVADAAKNTEICDELMTRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNFFVEKLLVTWKRVGLELKPHSSAECNFCRRPLHFEVMSEREKAYFSGMSKMVSAQA

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 57-642aa

Sequence Info: Full Length of Mature Protein

MW: 72.3 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one which is expressed in all tissues.

Reference: "BayGenomics: a resource of insertional mutations in mouse embryonic stem cells." Stryke D., Kawamoto M., Huang C.C., Johns S.J., King L.A., Harper C.A., Meng E.C., Lee R.E., Yee A., L'Italien L., Chuang P.T., Young S.G., Skarnes W.C., Babbitt P.C., Ferrin T.E. Nucleic Acids Res. 31:278-281(2003)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q8VC19

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A