Recombinant Moringa oleifera Chitin-binding protein 3, partial | CSB-EP311177MIJ

Recombinant Moringa oleifera Chitin-binding protein 3, partial | CSB-EP311177MIJ | Cusabio

Alternative Name(s): /

Gene Names: N/A

Research Areas: others

Organism: Moringa oleifera (Horseradish tree) (Moringa pterygosperma)

AA Sequence: CPAIQRCCQQLRNIQPPCRCCQ

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-22aa

Sequence Info: Full Length

MW: 29.2 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Chitin-binding protein (PubMed:23193603). Has antifungal activity against F.solani, F.oxysporum, C.musae and C.gloesporoides but not against P.oligandrum (PubMed:23193603). Depending on concentration the antifungal activity can be fungistatic or fungicidal (PubMed:23193603). Inhibits both spore germination and mycelial growth in F.solani at a concentration of 0.1 mg/ml (PubMed:23193603). Has antifungal activity against C.krusei, C.albicans, C.tropicalis and C.parapsilosis (PubMed:28634471). Has no chitinase, beta-glucanase or hemagglutinating activity (PubMed:23193603). Acts as a flocculent (PubMed:23193603).

Reference: "A Chitin-binding Protein Purified from Moringa oleifera Seeds Presents Anticandidal Activity by Increasing Cell Membrane Permeability and Reactive Oxygen Species Production." Neto J.X.S., Pereira M.L., Oliveira J.T.A., Rocha-Bezerra L.C.B., Lopes T.D.P., Costa H.P.S., Sousa D.O.B., Rocha B.A.M., Grangeiro T.B., Freire J.E.C., Monteiro-Moreira A.C.O., Lobo M.D.P., Brilhante R.S.N., Vasconcelos I.M. Front. Microbiol. 8:980-980(2017)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P86528

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A