Recombinant Microbacterium testaceum N-acyl homoserine lactonase (aiiM) | CSB-EP512020MTN

Recombinant Microbacterium testaceum N-acyl homoserine lactonase (aiiM) | CSB-EP512020MTN | Cusabio

Alternative Name(s): AHL-lactonase

Gene Names: aiiM

Research Areas: Others

Organism: Microbacterium testaceum (strain StLB037)

AA Sequence: MILAHDVSGSGPLLVLLHGITEDRRSWDPVDFTDGFTVVRVDLRGHGASAAEEPYDIPTLATDVHDTLAQLAENDVIPGELPVIVGHSMGGIVATAYGALFPARAIVNVDQPLQLAGMQGQVQQAEGMLRGADFPLFIHGMFAQMAGGLDAEELARVNGIRSPRQDVVLGMWRPLLEDSPEELAALVSGLTRIPEDVPYLVITGLDAGPEYAAWLQREIPQAVQEVWQPPTHYPHLVDPARFVERVEAFVR

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-251aa

Sequence Info: Full Length

MW: 43.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Hydrolyzes short- and long-chain N-acyl homoserine lactones with or without 3-oxo substitution at C3. Has slight activity towards L-homoserine lactone, and no activity towards gamma-butyrolactone.

Reference: "AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum."Wang W., Morohoshi T., Ikenoya M., Someya N., Ikeda T.Appl. Environ. Microbiol. 76:2524-2530(2010)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Hydrolyzes short- and long-chain N-acyl homoserine lactones with or without 3-oxo substitution at C3. Has slight activity towards L-homoserine lactone, and no activity towards gamma-butyrolactone.

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: C6L862

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A