Recombinant Lemna minor ATP synthase subunit beta, chloroplastic (atpB) | CSB-RP139994Pl

Recombinant Lemna minor ATP synthase subunit beta, chloroplastic (atpB) | CSB-RP139994Pl | Cusabio

Alternative Name(s): ATP synthase F1 sector subunit beta

Gene Names: atpB

Research Areas: Others

Organism: Lemna minor (Common duckweed)

AA Sequence: MQINPTTSGTAVSQLEEKNLGRVAQIIGPVLDVVFPPGKMPNIYNALVVKGQDADGQEIKVTCEVQQLLGNNRVRAVAMSATDGLTRGMDVIDTGAPLSVPVGGATLGRIFNVLGEPVDNLGPVDTRTTSPIHRSAPAFIQLDTKLAIFETGIKVVDLLAPYRRGGKIGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESGVINEKNITESKVALVYGQMNEPPGARMRVGLTALTMAEYFRDVNEQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLSTEMGSLQERITSTKEGSITSIQAVYVPADDLTDPAPATTFAHLDATTVLSRGLAAKGIYPAVDPLDSTSTMLQPGIVGEDHYETAQRVKETLQRYKELQDIIAILGLDELSEEDRLTVARARKIERFLSQPFFVAEVFTGSPGKYVGLEETIRGFKLILSGELDSLPEQAFYLVGNIDEATAKAINLEVESKLK

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-497aa

Sequence Info: Full Length

MW: 57.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Produces ATP from ADP in the presence of a proton gradient across the mbrane. The catalytic sites are hosted primarily by the beta subunits.

Reference: Complete sequence of the Duckweed (Lemna minor) chloroplast genome structural organization and phylogenetic relationships to other angiosperms.Mardanov A.V., Ravin N.V., Kuznetsov B.B., Samigullin T.H., Antonov A.S., Kolganova T.V., Skyabin K.G.J. Mol. Evol. 66:555-564(2008)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.

Involvement in disease:

Subcellular Location: Plastid, chloroplast thylakoid membrane, Peripheral membrane protein

Protein Families: ATPase alpha/beta chains family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: A9L9A3

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A