Recombinant Legionella pneumophila subsp. pneumophila Gamma-glutamyl phosphate reductase (proA) | CSB-EP726567LDL
- 13 - 23 Working Days
Recombinant Legionella pneumophila subsp. pneumophila Gamma-glutamyl phosphate reductase (proA) | CSB-EP726567LDL | Cusabio
Alternative Name(s): Gamma-glutamyl phosphate reductase(GPR)(EC 184.108.40.206)(Glutamate-5-semialdehyde dehydrogenase)(Glutamyl-gamma-semialdehyde dehydrogenase)(GSA dehydrogenase)
Gene Names: proA
Research Areas: Others
Organism: Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
AA Sequence: MNTNIANQLRAAKKATTDLNLIQSDTRTIILKTLAANLEKHIENIIQENQKDLSLMLEQDPRYDRLLLNKERILSLVNDVRKVANLPNPLGVNLFEKSMPNGLSIKKITVPLGVIAVIYESRPNVTIDIFSLCFKSGNVCILKGGKEAHFTNSYLLLLIKNTLKNFNINTDIVCLLPPERALMTQLLNATGLVDLCIPRGSQNLINFVRDNAKIPVIETGAGIVHTYFDKSGDLGKGKKIINNAKTRRVSVCNALDTLIIHADRLKDLPELVETLSQKNVIIYADQDAYHVLDKNYPEQLLIKAKPQDFGHEFLDYKLAIKTVPNIKAAIDHIQQFSSHHSEAVIAEDESAIDKFLTEVDAAAVYANASTAFTDGGEFGLGAEIGISTQKVHARGPMGLEALTSYKWVIRGTGQIRD
Tag Info: N-terminal 6xHis-tagged
Expression Region: 1-417aa
Sequence Info: Full Length
MW: 52.1 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Reference: "The genomic sequence of the accidental pathogen Legionella pneumophila." Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G., Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V., Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S. Russo J.J. Science 305:1966-1968(2004)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q5ZV33
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A