Recombinant Lampetra fluviatilis NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) | CSB-CF015076LNM(A4)

Recombinant Lampetra fluviatilis NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) | CSB-CF015076LNM(A4) | Cusabio

Alternative Name(s): NADH dehydrogenase subunit 1 MTND1, NADH1, ND1

Gene Names: MT-ND1

Research Areas: Others

Organism: Lampetra fluviatilis (European river lamprey) (Petromyzon fluviatilis)

AA Sequence: MLVMLTSTLILVLMVLLAVAFLTMVERKTLGYMQLRKGPNVVGFMGLLQPIADGVKLFLKEPVWPTAASPALFIAAPIMALTLALSLWMFIPMPQSISTINLTLLVILAISSLSVYASLGSGWASNSKYALIGALRAVAQTISYEVSLGLILLCLIILTGGFSLQAFIYTQEHTWFLLSSWPLAAMWFVSTLAETNRTPFDLTEGESELVSGFNVEYAGGPFALFFLAEYSNILFMNTLTAIMFLGPLGPNNLNILPIINVMMKATPLIILFLWIRASYPRFRYDQLMHLMWKNFLPLNLALFTLQLSLAVSLGGAGVPQM

Source: in vitro E.coli expression system

Tag Info: N-terminal 10xHis-SUMO-tagged

Expression Region: 1-321aa

Sequence Info: Full Length

MW: 53.8 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Reference: "The main features of the craniate mitochondrial DNA between the ND1 and the COI genes were established in the common ancestor with the lancelet." Delarbre C., Barriel V., Tillier S., Janvier P., Gachelin G. Mol. Biol. Evol. 14:807-813(1997)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

Involvement in disease:

Subcellular Location: Mitochondrion inner membrane, Multi-pass membrane protein

Protein Families: Complex I subunit 1 family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: O21069

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A