Recombinant Lactobacillus plantarum Bacteriocin plantaricin-A (plnA) | CSB-EP302126LMS

Recombinant Lactobacillus plantarum Bacteriocin plantaricin-A (plnA) | CSB-EP302126LMS | Cusabio

Alternative Name(s):

Gene Names: plnA

Research Areas: Others

Organism: Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)

AA Sequence: AYSLQMGATAIKQVKKLFKKWGW

Source: E.coli

Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Expression Region: 26-48aa

Sequence Info: Full Length of Mature Protein

MW: 18.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: This heat stable bacteriocin inhibits the growth of closely related Lactobacillus species. It may act as a pore-forming protein, creating a channel in the cell membrane through a 'barrel stave' mechanism.

Reference: "Structure and mode of action of the membrane-permeabilizing antimicrobial peptide pheromone plantaricin A." Kristiansen P.E., Fimland G., Mantzilas D., Nissen-Meyer J. J Biol Chem 280:22945-22950(2005)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P80214

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A