Recombinant Human Vesicle transport through interaction with t-SNAREs homolog 1B (VTI1B) , partial | CSB-EP887014HU

Recombinant Human Vesicle transport through interaction with t-SNAREs homolog 1B (VTI1B) , partial | CSB-EP887014HU | Cusabio

Alternative Name(s): Vesicle transport v-SNARE protein Vti1-like 1Vti1-rp1

Gene Names: VTI1B

Research Areas: Transport

Organism: Homo sapiens (Human)

AA Sequence: SSEHFEKLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLL

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 7-208aa

Sequence Info: Partial

MW: 39.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target mbrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.

Reference: A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways.Fischer von Mollard G., Stevens T.H.J. Biol. Chem. 273:2624-2630(1998)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.

Involvement in disease:

Subcellular Location: Early endosome membrane, Single-pass type IV membrane protein, Late endosome membrane, Single-pass type IV membrane protein, Lysosome membrane, Cytoplasmic granule, Recycling endosome membrane, Single-pass type IV membrane protein

Protein Families: VTI1 family

Tissue Specificity: Expressed in all tissues examined.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9UEU0

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM