Recombinant Human Tubulin-specific chaperone C (TBCC) | CSB-EP023227HU

Recombinant Human Tubulin-specific chaperone C (TBCC) | CSB-EP023227HU | Cusabio

Alternative Name(s): Tubulin-folding cofactor C ;CFC

Gene Names: TBCC

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: ESVSCSAAAVRTGDMESQRDLSLVPERLQRREQERQLEVERRKQKRQNQEVEKENSHFFVATFVRERAAVEELLERAESVERLEEAASRLQGLQKLINDSVFFLAAYDLRQGQEALARLQAALAERRRGLQPKKRFAFKTRGKDAASSTKVDAAPGIPPAVESIQDSPLPKKAEGDLGPSWVCGFSNLESQVLEKRASELHQRDVLLTELSNCTVRLYGNPNTLRLTKAHSCKLLCGPVSTSVFLEDCSDCVLAVACQQLRIHSTKDTRIFLQVTSRAIVEDCSGIQFAPYTWSYPEIDKDFESSGLDRSKNNWNDVDDFNWLARDMASPNWSILPEEERNIQW

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 2-345aa

Sequence Info: Full Length of Mature Protein

MW: 43 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Tubulin-folding protein; involved in the final step of the tubulin folding pathway.

Reference: Pathway leading to correctly folded beta-tubulin.Tian G., Huang Y., Rommelaere H., Vandekerckhove J., Ampe C., Cowan N.J.Cell 86:287-296(1996)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Tubulin-folding protein; involved in the final step of the tubulin folding pathway.

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: TBCC family

Tissue Specificity: Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level).

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q15814

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM