Recombinant Human Transcobalamin-1 (TCN1) | CSB-EP023317HU

Recombinant Human Transcobalamin-1 (TCN1) | CSB-EP023317HU | Cusabio

Alternative Name(s): Haptocorrin Short name: HC Protein R Transcobalamin I Short name: TC I Short name: TCI

Gene Names: TCN1

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: EICEVSEENYIRLKPLLNTMIQSNYNRGTSAVNVVLSLKLVGIQIQTLMQKMIQQIKYNVKSRLSDVSSGELALIILALGVCRNAEENLIYDYHLIDKLENKFQAEIENMEAHNGTPLTNYYQLSLDVLALCLFNGNYSTAEVVNHFTPENKNYYFGSQFSVDTGAMAVLALTCVKKSLINGQIKADEGSLKNISIYTKSLVEKILSEKKENGLIGNTFSTGEAMQALFVSSDYYNENDWNCQQTLNTVLTEISQGAFSNPNAAAQVLPALMGKTFLDINKDSSCVSASGNFNISADEPITVTPPDSQSYISVNYSVRINETYFTNVTVLNGSVFLSVMEKAQKMNDTIFGFTMEERSWGPYITCIQGLCANNNDRTYWELLSGGEPLSQGAGSYVVRNGENLEVRWSKY

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 24-433aa

Sequence Info: Full Length of Mature Protein

MW: 61.6 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach.

Reference: "Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin."Furger E., Frei D.C., Schibli R., Fischer E., Prota A.E.J. Biol. Chem. 288:25466-25476(2013)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Eukaryotic cobalamin transport proteins family

Tissue Specificity: Produced by the salivary glands of the oral cavity, in response to ingestion of food. Major constituent of secondary granules in neutrophils.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P20061

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM