Recombinant Human Thiamin pyrophosphokinase 1 (TPK1) | CSB-EP024103HU

Recombinant Human Thiamin pyrophosphokinase 1 (TPK1) | CSB-EP024103HU | Cusabio

Alternative Name(s): Placental protein 20

Gene Names: TPK1

Research Areas: Others

Organism: Homo sapiens (Human)

AA Sequence: MEHAFTPLEPLLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCMQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-243aa

Sequence Info: Full Length

MW: 54.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.

Reference: "Isolation and characterization of a human thiamine pyrophosphokinase cDNA." Nosaka K., Onozuka M., Kakazu N., Hibi S., Nishimura H., Nishino H., Abe T. Biochim. Biophys. Acta 1517:293-297(2001)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.

Involvement in disease: Thiamine metabolism dysfunction syndrome 5, episodic encephalopathy type (THMD5)

Subcellular Location:

Protein Families: Thiamine pyrophosphokinase family

Tissue Specificity: Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9H3S4

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM