Recombinant Human Ribonuclease UK114 (HRSP12) | CSB-EP010744HU

Recombinant Human Ribonuclease UK114 (HRSP12) | CSB-EP010744HU | Cusabio

Alternative Name(s): 14.5KDA translational inhibitor protein ;p14.5Heat-responsive protein 12UK114 antigen homolog

Gene Names: HRSP12

Research Areas: Epigenetics and Nuclear Signaling

Organism: Homo sapiens (Human)

AA Sequence: SSLIRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGEILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIEAVAIQGPLTTASL

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 2-137AA

Sequence Info: Full Length of Mature Protein

MW: 41.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Endoribonuclease responsible for the inhibition of the translation by cleaving mRNA. Inhibits cell-free protein synthesis. Cleaves phosphodiester bonds only in single-stranded RNA .

Reference: Isolation and characterization of a 14.5-KDA trichloroacetic-acid-soluble translational inhibitor protein from human monocytes that is upregulated upon cellular differentiation.Schmiedeknecht G., Kerkhoff C., Orso E., Stoehr J., Aslanidis C., Nagy G.M., Knuechel R., Schmitz G.Eur. J. Biochem. 242:339-351(1996)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.

Involvement in disease:

Subcellular Location: Cytoplasm, Nucleus, Peroxisome, Mitochondrion

Protein Families: RutC family

Tissue Specificity: Expressed predominantly in liver and kidney. Lower levels in lung and brain.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P52758

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM