Recombinant Human Proheparin-binding EGF-like growth factor [Cleaved into: Heparin-binding EGF-like growth factor protein (HBEGF) (Active) | CSB-AP002651HU

Recombinant Human Proheparin-binding EGF-like growth factor [Cleaved into: Heparin-binding EGF-like growth factor protein (HBEGF) (Active) | CSB-AP002651HU | Cusabio

Protein Description: Full Length of Mature Protein

Alternative Name (s) : HB-EGF, Diphtheria toxin receptor, DT-R,],

Gene Names: HBEGF,DTR,DTS,HEGFL

Research Areas: Cancer

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 63-148aa

Sequence Info: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of ＞ 1.0 × 106 IU/mg.

MW: 9.7 kDa

Purity: >97% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

PubMed ID: 1840698; 14702039; 15372022; 15489334; 1556128; 7836353; 9135143; 16508205; 22171320; 23234360; 9659904

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Involvement in disease:

Subcellular Location: Heparin-binding EGF-like growth factor: Secreted, extracellular space

Protein Families:

Tissue Specificity:

Paythway: ErbBsignalingpathway

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130 mM NaCl

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q99075

Uniprot Entry Name: HBEGF_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM