Recombinant Human Profilin-1 (PFN1) | CSB-EP017824HU

Recombinant Human Profilin-1 (PFN1) | CSB-EP017824HU | Cusabio

Alternative Name(s): Epididymis tissue protein Li 184aProfilin I

Gene Names: PFN1

Research Areas: Neuroscience

Organism: Homo sapiens (Human)

AA Sequence: AGWNAYIDNLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVNITPAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVTVTKTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 2-140aa

Sequence Info: Full Length of Mature Protein

MW: 41.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

Reference: Human profilin. Molecular cloning, sequence comparison, and chromosomal analysis.Kwiatkowski D.J., Bruns G.A.P.J. Biol. Chem. 263:5910-5915(1988)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

Involvement in disease: Amyotrophic lateral sclerosis 18 (ALS18)

Subcellular Location: Cytoplasm, cytoskeleton

Protein Families: Profilin family

Tissue Specificity: Expressed in epididymis (at protein level).

Paythway: Regulationofactincytoskeleton

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P07737

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM