Recombinant Human Probable dimethyladenosine transferase (DIMT1) | CSB-EP892161HU

Recombinant Human Probable dimethyladenosine transferase (DIMT1) | CSB-EP892161HU | Cusabio

Alternative Name(s): DIM1 dimethyladenosine transferase 1 homologDIM1 dimethyladenosine transferase 1-likeProbable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferaseProbable 18S rRNA dimethylaseProbable S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Gene Names: DIMT1

Research Areas: Epigenetics and Nuclear Signaling

Organism: Homo sapiens (Human)

AA Sequence: MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-313aa

Sequence Info: Full Length

MW: 51.2 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.

Reference: Full-insert sequence of mapped XREF EST.Barrow I.K.-P., Boguski M.S., Touchman J.W., Spencer F.Functional proteomic analysis of human nucleolus.Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.Mol. Biol. Cell 13:4100-4109(2002)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle

Involvement in disease:

Subcellular Location: Nucleus, nucleoplasm, Nucleus, nucleolus

Protein Families: Class I-like SAM-binding methyltransferase superfamily, rRNA adenine N(6)-methyltransferase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9UNQ2

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM