Cusabio Active Proteins
Recombinant Human Pro-epidermal growth factor (EGF), partial (Active) | CSB-AP003711HU
- 5 to 10 Working Days
Recombinant Human Pro-epidermal growth factor (EGF) ,partial (Active) | CSB-AP003711HU | Cusabio
Protein Description: Partial
Alternative Name (s) : Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
Gene Names: EGF
Research Areas: Signal Transduction
Species: Homo sapiens (Human)
Tag Info: Tag-Free
Expression Region: 971-1023aa
Sequence Info: NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR
Biological Activity: The ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 cells is typically 0.1-0.5 ng/ml
MW: 6.2 kDa
Purity: Greater than 95% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU/µg as determined by LAL method.
Relevance: Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha) , which is a competitor for EGF receptor sites.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Function: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro
Involvement in disease: Hypomagnesemia 4 (HOMG4)
Subcellular Location: Membrane, Single-pass type I membrane protein
Tissue Specificity: Expressed in kidney, salivary gland, cerebrum and prostate.
Form: Lyophilized powder
Buffer: Lyophilized from a 0.2 μm Filtered 20 mM Tris-HCl, 200 mM NaCl, pH 8.0
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P01133
Uniprot Entry Name:
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM