Recombinant Human Pleckstrin homology domain-containing family F member 2 (PLEKHF2) | CSB-EP884492HU

Recombinant Human Pleckstrin homology domain-containing family F member 2 (PLEKHF2) | CSB-EP884492HU | Cusabio

Alternative Name(s): Endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains

Gene Names: PLEKHF2

Research Areas: Cell Biology

Organism: Homo sapiens (Human)

AA Sequence: MVDRLANSEANTRRISIVENCFGAAGQPLTIPGRVLIGEGVLTKLCRKKPKARQFFLFNDILVYGNIVIQKKKYNKQHIIPLENVTIDSIKDEGDLRNGWLIKTPTKSFAVYAATATEKSEWMNHINKCVTDLLSKSGKTPSNEHAAVWVPDSEATVCMRCQKAKFTPVNRRHHCRKCGFVVCGPCSEKRFLLPSQSSKPVRICDFCYDLLSAGDMATCQPARSDSYSQSLKSPLNDMSDDDDDDDSSD

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-249aa

Sequence Info: Full Length

MW: 54.8 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis

Reference: "Phafin 2, PH and FYVE domain-containing protein 2." Shi H., Hong W. Submitted (OCT-2001)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis

Involvement in disease:

Subcellular Location: Early endosome membrane, Peripheral membrane protein, Endoplasmic reticulum

Protein Families:

Tissue Specificity: Expressed in placenta, ovary and small intestine, as well as in heart and pancreas. Also expressed in peripheral blood mononuclear cells and dendritic cells.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9H8W4

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM