Recombinant Human Phosphoglycerate kinase 1 (PGK1), partial | CSB-EP017856HU1

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3 - 7 Working Days
  • Recombinant Human Phosphoglycerate kinase 1 (PGK1), partial
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
€245.00 - €1,277.00
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Recombinant Human Phosphoglycerate kinase 1 (PGK1), partial | CSB-EP017856HU1 | Cusabio

Alternative Name(s): Cell migration-inducing gene 10 protein;Primer recognition protein 2 ;PRP 2

Gene Names: PGK1

Research Areas: Metabolism

Organism: Homo sapiens (Human)


Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 25-417aa

Sequence Info: Partial

MW: 68.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: In addition to its role as a glycolytic enzyme, it ses that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).

Reference: Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase.Michelson A.M., Markham A.F., Orkin S.H.Proc. Natl. Acad. Sci. U.S.A. 80:472-476(1983)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein)

Involvement in disease: Phosphoglycerate kinase 1 deficiency (PGK1D)

Subcellular Location: Cytoplasm

Protein Families: Phosphoglycerate kinase family

Tissue Specificity: Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.

Paythway: HIF-1signalingpathway

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P00558

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM

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