Recombinant Human PDZ and LIM domain protein 3 (PDLIM3) | CSB-EP690477HU

Recombinant Human PDZ and LIM domain protein 3 (PDLIM3) | CSB-EP690477HU | Cusabio

Alternative Name(s): Actinin-associated LIM protein;Alpha-actinin-2-associated LIM protein

Gene Names: PDLIM3

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQKTGTTLNTSIIFGPNLS

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-189aa

Sequence Info: Full Length of Isoform 3

MW: 36.2 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: May play a role in the organization of actin filament arrays within muscle cells.

Reference: Actinin-associated LIM protein identification of a domain interaction between PDZ and spectrin-like repeat motifs.Xia H., Winokur S.T., Kuo W.-L., Altherr M.R., Bredt D.S.J. Cell Biol. 139:507-515(1997)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: May play a role in the organization of actin filament arrays within muscle cells.

Involvement in disease:

Subcellular Location: Cytoplasm, myofibril, sarcomere, Z line

Protein Families:

Tissue Specificity: Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q53GG5

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM