Recombinant Human LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) | CSB-EP232922HU

Recombinant Human LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) | CSB-EP232922HU | Cusabio

Alternative Name(s): Particularly interesting new Cys-His protein 1

Gene Names: LIMS1

Research Areas: Cardiovascular

Organism: Homo sapiens (Human)

AA Sequence: MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFTIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGGVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-325aa

Sequence Info: Full Length of BC005341

MW: 64.2 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.

Reference: "A new LIM protein containing an autoepitope homologous to 'senescent cell antigen'." Rearden A. Biochem. Biophys. Res. Commun. 201:1124-1131(1994)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.

Involvement in disease:

Subcellular Location: Cell junction, focal adhesion, Cell membrane, Peripheral membrane protein, Cytoplasmic side

Protein Families:

Tissue Specificity: Expressed in most tissues except in the brain.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P48059

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM