Recombinant Human KeRatin, type I cytoskeletal 18 (KRT18), partial | CSB-EP012519HU1

Recombinant Human KeRatin, type I cytoskeletal 18 (KRT18), partial | CSB-EP012519HU1 | Cusabio

Alternative Name(s): Cell proliferation-inducing gene 46 protein;Cytokeratin-18 ;CK-18Keratin-18 ;K18

Gene Names: KRT18

Research Areas: Cell Cycle

Organism: Homo sapiens (Human)

AA Sequence: TNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 11-430aa

Sequence Info: Partial

MW: 50.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells . When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma mbrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection

Reference: Comparison of mouse and human keratin 18 a component of intermediate filaments expressed prior to implantation.Oshima R.G., Millan J.L., Cecena G.Differentiation 33:61-68(1986)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Involvement in disease: Cirrhosis (CIRRH)

Subcellular Location: Cytoplasm, perinuclear region, Nucleus, nucleolus

Protein Families: Intermediate filament family

Tissue Specificity: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

Paythway: Estrogensignalingpathway

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P05783

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM