Recombinant Human Guanine nucleotide-binding protein G (s) subunit alpha isoforms short (GNAS) | CSB-EP352389HU

Recombinant Human Guanine nucleotide-binding protein G (s) subunit alpha isoforms short (GNAS) | CSB-EP352389HU | Cusabio

Alternative Name(s): Adenylate cyclase-stimulating G alpha protein

Gene Names: GNAS

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-394aa

Sequence Info: Full Length

MW: 61.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) . Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP . GNAS functions downstream of several GPCRs, including beta-adrenergic receptors . Stimulates the Ras signaling pathway via RAPGEF2 .

Reference: "Identification by molecular cloning of two forms of the alpha-subunit of the human liver stimulatory (GS) regulatory component of adenylyl cyclase."Mattera R., Codina J., Crozat A., Kidd V., Woo S.L.C., Birnbaumer L.FEBS Lett. 206:36-42(1986)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs)

Involvement in disease: Albright hereditary osteodystrophy (AHO); Pseudohypoparathyroidism 1A (PHP1A); McCune-Albright syndrome (MAS); Progressive osseous heteroplasia (POH); ACTH-independent macronodular adrenal hyperplasia 1 (AIMAH1); Pseudohypoparathyroidism 1B (PHP1B); GNAS hyperfunction (GNASHYP); Pseudohypoparathyroidism 1C (PHP1C)

Subcellular Location: Cell membrane, Lipid-anchor

Protein Families: G-alpha family, G(s) subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P63092

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: OMIM