Recombinant Human Grancalcin (GCA) | CSB-EP009307HU

Recombinant Human Grancalcin (GCA) | CSB-EP009307HU | Cusabio

Alternative Name(s): GCA; GCL; Grancalcin

Gene Names: GCA

Research Areas: Others

Organism: Homo sapiens (Human)

AA Sequence: MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-217aa

Sequence Info: Full Length

MW: 51 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.

Reference: "Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner." Lollike K., Johnsen A.H., Durussel I., Borregaard N., Cox J.A. J. Biol. Chem. 276:17762-17769(2001)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.

Involvement in disease:

Subcellular Location: Cytoplasm, Cytoplasmic granule membrane, Peripheral membrane protein, Cytoplasmic side

Protein Families:

Tissue Specificity: Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P28676

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM