Recombinant Human Glucosidase 2 subunit beta (PRKCSH), partial | CSB-RP029254h

Recombinant Human Glucosidase 2 subunit beta (PRKCSH), partial | CSB-RP029254h | Cusabio

Alternative Name(s): 80K-H protein;Glucosidase II subunit betaProtein kinase C substrate 60.1KDA protein heavy chain ;PKCSH

Gene Names: PRKCSH

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: VEVKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYRSEALPTDLPAPSAPDLTEPKE

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 15-302aa

Sequence Info: Partial

MW: 59.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Regulatory subunit of glucosidase II.

Reference: Isolation of cDNAs encoding a substrate for protein kinase C nucleotide sequence and chromosomal mapping of the gene for a human 80K protein.Sakai K., Masamichi H., Minoshima S., Kudoh J., Fukuyama R., Shimizu N.Genomics 5:309-315(1989)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Regulatory subunit of glucosidase II.

Involvement in disease: Polycystic liver disease 1 (PCLD1)

Subcellular Location: Endoplasmic reticulum

Protein Families:

Tissue Specificity:

Paythway: Proteinprocessinginendoplasmicreticulum

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P14314

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM