Recombinant Human Epiplakin (EPPK1) , partial | CSB-EP007745HU

Recombinant Human Epiplakin (EPPK1) , partial | CSB-EP007745HU | Cusabio

Alternative Name(s): 450KDA epidermal antigen

Gene Names: EPPK1

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: MSGHTLPPLPVPGTNSTEQASVPRAMAATLGAGTPPRPQARSIAGVYVEASGQAQSVYAAMEQGLLPAGLGQALLEAQAATGGLVDLARGQLLPVSKALQQGLVGLELKEKLLAAERATTGYPDPYGGEKLALFQAIGKEVVDRALGQSWLEVQLATGGLVDPAQGVLVAPEPACHQGLLDRETWHKLSELEPGTGDLRFLNPNTLERLTYHQLLERCVRAPGSG

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-225aa

Sequence Info: Partial

MW: 27.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance:

Reference: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Cytoskeletal linker protein that connects to intermediate filaments and controls their reorganization in response to stress

Involvement in disease:

Subcellular Location: Cytoplasm, cytoskeleton, Cell junction, hemidesmosome, Cell junction, tight junction, Cell projection, Apicolateral cell membrane, Basolateral cell membrane, Cell junction

Protein Families: Plakin or cytolinker family

Tissue Specificity: Expressed in epithelial cells of liver, small intestine, colon, salivary glands, stomach and appendix.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P58107

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM