Recombinant Human Dynactin subunit 6 (DCTN6) | CSB-EP006569HU

Recombinant Human Dynactin subunit 6 (DCTN6) | CSB-EP006569HU | Cusabio

Alternative Name(s): Dynactin subunit p27 Protein WS-3

Gene Names: DCTN6

Research Areas: Cell Biology

Organism: Homo sapiens (Human)

AA Sequence: MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVIGEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMKMGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLRRVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-190aa

Sequence Info: Full Length

MW: 47.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance:

Reference: "Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit." Yeh T.Y., Kowalska A.K., Scipioni B.R., Cheong F.K., Zheng M., Derewenda U., Derewenda Z.S., Schroer T.A. EMBO J. 32:1023-1035(2013)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location: Cytoplasm, cytoskeleton, Chromosome, centromere, kinetochore

Protein Families: Dynactin subunits 5/6 family, Dynactin subunit 6 subfamily

Tissue Specificity: Ubiquitous.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: O00399

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM