Recombinant Human DnaJ homolog subfamily B member 2 (DNAJB2) | CSB-EP007008HU

Recombinant Human DnaJ homolog subfamily B member 2 (DNAJB2) | CSB-EP007008HU | Cusabio

Alternative Name(s): DnaJ protein homolog 1Heat shock 40KDA protein 3Heat shock protein J1 ;HSJ-1

Gene Names: DNAJB2

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: ASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGGREAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 2-324aa

Sequence Info: Full Length of Isoform 3

MW: 51.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance:

Reference: A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.Blumen S.C., Astord S., Robin V., Vignaud L., Toumi N., Cieslik A., Achiron A., Carasso R.L., Gurevich M., Braverman I., Blumen N., Munich A., Barkats M., Viollet L.Ann. Neurol. 71:509-519(2012)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family

Involvement in disease: Distal spinal muscular atrophy, autosomal recessive, 5 (DSMA5)

Subcellular Location: Isoform 2: Cytoplasm, Nucleus, SUBCELLULAR LOCATION: Isoform 1: Endoplasmic reticulum membrane, Lipid-anchor, Cytoplasmic side

Protein Families:

Tissue Specificity: More abundantly expressed in neocortex, cerebellum, spinal cord and retina where it is expressed by neuronal cells (at protein level) (PubMed:1599432, PubMed:12754272). Detected at much lower level in non-neuronal tissues including kidney, lung, heart, skeletal muscle, spleen and testis (at protein level) (PubMed:12754272, PubMed:1599432). Isoform 1 is more abundant in neocortex and cerebellum compared to isoform 2 (at protein level) (PubMed:12754272).

Paythway: Proteinprocessinginendoplasmicreticulum

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P25686

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM