Recombinant Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial (BDH1) | CSB-EP002648HU

Recombinant Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial (BDH1) | CSB-EP002648HU | Cusabio

Alternative Name(s): 3-hydroxybutyrate dehydrogenase;Short chain dehydrogenase/reductase family 9C member 1

Gene Names: BDH1

Research Areas: Cancer

Organism: Homo sapiens (Human)

AA Sequence: ASAAEPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLGVKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHLPGAISDMIYIR

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 47-343aa

Sequence Info: Full Length of Mature Protein

MW: 37.1 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance:

Reference: Molecular cloning and characterization of (R)-3-hydroxybutyrate dehydrogenase from human heart.Marks A.R., McIntyre J.O., Duncan T.M., Erdjument-Bromage H., Tempst P., Fleischer S.J. Biol. Chem. 267:15459-15463(1992)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location: Mitochondrion inner membrane, Mitochondrion matrix

Protein Families: Short-chain dehydrogenases/reductases (SDR) family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q02338

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM