Recombinant Human Carbonic anhydrase 12 (CA12), partial | CSB-EP004367HU
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Recombinant Human Carbonic anhydrase 12 (CA12), partial | CSB-EP004367HU | Cusabio
Alternative Name(s): Carbonate dehydratase XII;Carbonic anhydrase XII ;CA-XIITumor antigen HOM-R;CC-3.1.3
Gene Names: CA12
Research Areas: Cancer
Organism: Homo sapiens (Human)
AA Sequence: APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 25-301aa
Sequence Info: Extracellular Domain
MW: 47.1 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Reversible hydration of carbon dioxide.
Reference: Human carbonic anhydrase XII cDNA cloning, expression, and chromosomal localization of a carbonic anhydrase gene that is overexpressed in some renal cell cancers.Tuereci O., Sahin U., Vollmar E., Siemer S., Goettert E., Seitz G., Parkkila A.-K., Shah G.N., Grubb J.H., Pfreundschuh M., Sly W.S.Proc. Natl. Acad. Sci. U.S.A. 95:7608-7613(1998)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Reversible hydration of carbon dioxide.
Involvement in disease: Hyperchlorhidrosis, isolated (HCHLH)
Subcellular Location: Membrane, Single-pass type I membrane protein
Protein Families: Alpha-carbonic anhydrase family
Tissue Specificity: Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: O43570
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM