Recombinant Human Beta-defensin 1 protein (DEFB1), partial (Active) | CSB-AP003031HU

Recombinant Human Beta-defensin 1 protein (DEFB1) ,partial (Active) | CSB-AP003031HU | Cusabio

Protein Description: Partial

Alternative Name (s) : BD-1, Defensin, beta 1

Gene Names: DEFB1,BD1,HBD1

Research Areas: Microbiology

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 22-68aa

Sequence Info: GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK

Biological Activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml.

MW: 5.1 kDa

Purity: >98% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Has bactericidal activity. {ECO:0000250}.

PubMed ID: 9268634; 9070620; 15489334; 7628632; 12010514; 11486002; 11714914

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility

Involvement in disease:

Subcellular Location: Secreted, Membrane

Protein Families: Beta-defensin family

Tissue Specificity: Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level) .

Paythway:

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130 mM NaCl

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P60022

Uniprot Entry Name: DEFB1_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM