Recombinant Human Ameloblastin (AMBN) | CSB-YP882061HU

Recombinant Human Ameloblastin (AMBN) | CSB-YP882061HU | Cusabio

Alternative Name(s): AMBN; AMBN_HUMAN; Ameloblastin (enamel matrix protein); Ameloblastin

Gene Names: AMBN

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: VPFFPQQSGTPGMASLSLETMRQLGSLQRLNTLSQYSRYGFGKSFNSLWMHGLLPPHSSLPWMRPREHETQQYEYSLPVHPPPLPSQPSLKPQQPGLKPFLQSAAATTNQATALKEALQPPIHLGHLPLQEGELPLVQQQVAPSDKPPKPELPGVDFADPQGPSLPGMDFPDPQGPSLPGLDFADPQGSTIFQIARLISHGPMPQNKQSPLYPGMLYVPFGANQLNAPARLGIMSSEEVAGGREDPMAYGAMFPGFGGMRPGFEGMPHNPAMGGDFTLEFDSPVAATKGPENEEGGAQGSPMPEANPDNLENPAFLTELEPAPHAGLLALPKDDIPGLPRSPSGKMKGLPSVTPAAADPLMTPELADVYRTYDADMTTSVDFQEEATMDTTMAPNSLQTSMPGNKAQEPEMMHDAWHFQEP

Source: Yeast

Tag Info: N-terminal 6xHis-tagged

Expression Region: 27-447aa

Sequence Info: Full Length of Mature Protein

MW: 47.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Involved in the mineralization and structural organization of enamel.

Reference: "Cloning, characterization and immunolocalization of human ameloblastin."MacDougall M., Simmons D., Gu T.T., Forsman-Semb K., Mardh C.K., Mesbah M., Forest N., Krebsbach P.H., Yamada Y., Berdal A.Eur. J. Oral Sci. 108:303-310(2000)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Involved in the mineralization and structural organization of enamel.

Involvement in disease: Amelogenesis imperfecta 1F (AI1F)

Subcellular Location: Secreted, extracellular space, extracellular matrix

Protein Families: Ameloblastin family

Tissue Specificity: Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9NP70

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM