Recombinant Human Aldehyde dehydrogenase family 1 member A3 (ALDH1A3) | CSB-EP001567HU

Recombinant Human Aldehyde dehydrogenase family 1 member A3 (ALDH1A3) | CSB-EP001567HU | Cusabio

Alternative Name(s): Aldehyde dehydrogenase 6 Retinaldehyde dehydrogenase 3 Short name:RALDH-3 Short name:RalDH3

Gene Names: ALDH1A3

Research Areas: Others

Organism: Homo sapiens (Human)

AA Sequence: ATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDDNVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVTIKLGDKNP

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 2-512aa

Sequence Info: Full Length of Mature Protein

MW: 72 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system (By similarity).

Reference: "Molecular cloning, genomic organization, and chromosomal localization of an additional human aldehyde dehydrogenase gene, ALDH6." Hsu L.C., Chang W.-C., Hiraoka L., Hsieh C.-L.Genomics 24:333-341(1994)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: NAD-dependent aldehyde dehydrogenase that catalyzes the formation of retinoic acid

Involvement in disease: Microphthalmia, isolated, 8 (MCOP8)

Subcellular Location: Cytoplasm

Protein Families: Aldehyde dehydrogenase family

Tissue Specificity: Expressed at low levels in many tissues and at higher levels in salivary gland, stomach, and kidney.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P47895

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM