Recombinant Human Alcohol dehydrogenase class-3 (ADH5) | CSB-EP001357HU

Recombinant Human Alcohol dehydrogenase class-3 (ADH5) | CSB-EP001357HU | Cusabio

Alternative Name(s): Alcohol dehydrogenase 5;Alcohol dehydrogenase class chi chain;Alcohol dehydrogenase class-III;Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-) ;FALDH ;FDH ;GSH-FDHS-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)

Gene Names: ADH5

Research Areas: Metabolism

Organism: Homo sapiens (Human)

AA Sequence: ANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 2-374aa

Sequence Info: Full Length of Mature Protein

MW: 66.6 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Class-III ADH is rarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.

Reference: cDNA sequence of human class III alcohol dehydrogenase.Sharma C.P., Fox E.A., Holmquist B., Joernvall H., Vallee B.L.Biochem. Biophys. Res. Commun. 164:631-637(1989)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: Zinc-containing alcohol dehydrogenase family, Class-III subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P11766

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM