Cusabio Human Recombinants
Recombinant Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) | CSB-EP861152HU
- 3 - 7 Working Days
Recombinant Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) | CSB-EP861152HU | Cusabio
Alternative Name(s): Acetyl-CoA transferase-like protein Cytosolic acetoacetyl-CoA thiolase
Gene Names: ACAT2
Research Areas: Metabolism
Organism: Homo sapiens (Human)
AA Sequence: MNAGSDPVVIVSAARTIIGSFNGALAAVPVQDLGSTVIKEVLKRATVAPEDVSEVIFGHVLAAGCGQNPVRQASVGAGIPYSVPAWSCQMICGSGLKAVCLAVQSIGIGDSSIVVAGGMENMSKAPHLAYLRTGVKIGEMPLTDSILCDGLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRKGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGTVTPANASGINDGAAAVVLMKKSEADKRGLTPLARIVSWSQVGVEPSIMGIGPIPAIKQAVTKAGWSLEDVDIFEINEAFAAVSAAIVKELGLNPEKVNIEGGAIALGHPLGASGCRILVTLLHTLERMGRSRGVAALCIGGGMGIAMCVQRE
Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression Region: 1-397aa
Sequence Info: Full Length
MW: 48.8 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Involved in the biosynthetic pathway of cholesterol.
Reference: "High resolution crystal structures of human cytosolic thiolase (CT): a comparison of the active sites of human CT, bacterial thiolase, and bacterial KAS I." Kursula P., Sikkila H., Fukao T., Kondo N., Wierenga R.K. J. Mol. Biol. 347:189-201(2005)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q9BWD1
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A