Recombinant Human 10KDA heat shock protein, mitochondrial (HSPE1) | CSB-RP108574h
- 13 - 23 Working Days
Recombinant Human 10KDA heat shock protein, mitochondrial (HSPE1) | CSB-RP108574h | Cusabio
Alternative Name(s): 10KDA chaperonin;Chaperonin 10 ;CPN10Early-pregnancy factor ;EPF
Gene Names: HSPE1
Research Areas: Neuroscience
Organism: Homo sapiens (Human)
AA Sequence: AGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD
Tag Info: N-terminal 6xHis-tagged
Expression Region: 2-102aa
Sequence Info: Full Length of Mature Protein
MW: 14.8 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
Reference: Identification and cloning of human chaperonin 10 homologue.Monzini N., Legname G., Marcucci F., Gromo G., Modena D.Biochim. Biophys. Acta 1218:478-480(1994)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix
Involvement in disease:
Subcellular Location: Mitochondrion matrix
Protein Families: GroES chaperonin family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P61604
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM