Recombinant Glycine max Seed biotin-containing protein SBP65 (SBP65) | CSB-EP661207GGV

Recombinant Glycine max Seed biotin-containing protein SBP65 (SBP65) | CSB-EP661207GGV | Cusabio

Alternative Name(s): BP75 Seed biotinylated protein of 65KDA

Gene Names: SBP65

Research Areas: Microbiology

Organism: Glycine max (Soybean) (Glycine hispida)

AA Sequence: MASEQLARRENTTTEKEIHVEKHRVPKMATHFEHLAEQAKESDITAGKDTPQGSIEALQAGERVKDHAGKAMGDIGGRGKARETHELGAHFESLADKVTDHAAANVVGNKESQREARGGVRDVGKFEMRTEGGEKGNKDRPELKTRTREVIGRTEKERGRESGGQVVAEKGRETETARGRVGAENEGARTTAVITCTLEKGGGTQKPIREEERESESERSAWEQISNYSDQATQGVKEKYERAKQAASETLNTTT

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-255aa

Sequence Info: Full Length

MW: 44 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: May serve as a biotin provider for several growth-limiting enzymes that are necessary during seed development and the subsequent germination stages, and thus may play some roles in determining seed germination capacity.

Reference: "Tissue- and stage-specific expression of a soybean (Glycine max L.) seed-maturation, biotinylated protein."Hsing Y.-C., Tsou C.-H., Hsu T.-F., Chen Z.-Y., Hsieh K.-L., Hsieh J.-S., Chow T.-Y.Plant Mol. Biol. 38:481-490(1998)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: May serve as a biotin provider for several growth-limiting enzymes that are necessary during seed development and the subsequent germination stages, and thus may play some roles in determining seed germination capacity.

Involvement in disease:

Subcellular Location:

Protein Families: Seed biotin-containing protein SBP65 family

Tissue Specificity: Expressed in the leaf primodium and the vascular tissues of the hypocotyl-radicle axis of mature seeds. High protein levels in dry and mature soybean seeds, but not found in fresh immature seeds.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q39846

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A