Recombinant Giardia lamblia virus Major capsid protein (gag) , partial | CSB-EP724163GBAS

Recombinant Giardia lamblia virus Major capsid protein (gag) , partial | CSB-EP724163GBAS | Cusabio

Alternative Name(s): Gag protein Major coat protein

Gene Names: gag

Research Areas: Microbiology

Organism: Giardia lamblia virus (isolate Wang) (GLV)

AA Sequence: MVWGTGYGNPSPLNPYGFASLHRGGLNPLILAPENITFDTLNTHNDHEETHGESPEVPKASIAPAGRQNVPVLQQNQENEDNHALGGSEDAKDEREIRFSAIKTLYNYYSEGPSTPIMPHLVNRLRGLDALAKIDATLTKVDMNAAYIFALRPTFPYSYGYKQRFSNRRLTTSALCYARTGLSSFLTVDKTYTSNSPLKGGSRGWP

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-206aa

Sequence Info: Partial

MW: 26.8 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Capsid protein self-assembles to form an icosahedral capsid with a T=2 symmetry, 35 nm in diameter, and consisting of 60 capsid proteins asymmetric dimers. The capsid encapsulates the genomic dsRNA and the polymerase and remains intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of the virion and are extruded into the cytoplasm (By similarity).

Reference: "Giardiavirus double-stranded RNA genome encodes a capsid polypeptide and a gag-pol-like fusion protein by a translation frameshift."Wang A.L., Yang H.M., Shen K.A., Wang C.C.Proc. Natl. Acad. Sci. U.S.A. 90:8595-8599(1993).

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Capsid protein self-assembles to form an icosahedral capsid with a T=2 symmetry, 35 nm in diameter, and consisting of 60 capsid proteins asymmetric dimers. The capsid encapsulates the genomic dsRNA and the polymerase and remains intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of the virion and are extruded into the cytoplasm (By similarity).

Involvement in disease:

Subcellular Location: Virion

Protein Families: Totivirus major capsid protein family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q67652

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A