Recombinant Geobacillus stearothermophilus ATP-dependent DNA helicase pcrA (pcrA) , partial | CSB-EP349564GFM

Recombinant Geobacillus stearothermophilus ATP-dependent DNA helicase pcrA (pcrA) , partial | CSB-EP349564GFM | Cusabio

Alternative Name(s): pcrA; ATP-dependent DNA helicase PcrA; EC 3.6.4.12

Gene Names: pcrA

Research Areas: Others

Organism: Geobacillus stearothermophilus (Bacillus stearothermophilus)

AA Sequence: AHLNKEQQEAVRTTEGPLLIMAGAGSGKTRVLTHRIAYLMAEKHVAPWNILAITFTNKAAREMRERVQSLLGGAAEDVWISTFHSMCVRILRRDIDRIGINRNFSILDPTDQLSVMKTILKEKNIDPKKFEPRTILGTISAAKNELLPPEQFAKRASTYYEKVVSDVYQEYQQRLLRNHSLDFDDLIMTTIQLFDRVPDVLHYYQYKFQYIHIDEYQDTNRAQYTLVKKLAERFQNICAVGDADQSIYRWRGADIQNILSFERDYPNAKVILLEQNYRST

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 10-289aa

Sequence Info: Partial

MW: 48.6 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.

Reference: "Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism."Bird L.E., Brannigan J.A., Subramanya H.S., Wigley D.B.Nucleic Acids Res. 26:2686-2693(1998)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.

Involvement in disease:

Subcellular Location:

Protein Families: Helicase family, UvrD subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P56255

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A