Recombinant Escherichia coli Oxidoreductase YdhF (ydhF) | CSB-EP300544ENV

Recombinant Escherichia coli Oxidoreductase YdhF (ydhF) | CSB-EP300544ENV | Cusabio

Alternative Name(s): ydhF; b1647; JW1639; Oxidoreductase YdhF; EC 1.-.-.-

Gene Names: ydhF

Research Areas: Others

Organism: Escherichia coli (strain K12)

AA Sequence: MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDLGVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITDRDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNFTPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLFNDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAETLKMTRQQWFRIRKAALGYDVP

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-298aa

Sequence Info: Full Length

MW: 37.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: May function as oxidoreductase.

Reference: "Structural genomics of highly conserved microbial genes of unknown function in search of new antibacterial targets." Abergel C., Coutard B., Byrne D., Chenivesse S., Claude J.-B., Deregnaucourt C., Fricaux T., Gianesini-Boutreux C., Jeudy S., Lebrun R., Maza C., Notredame C., Poirot O., Suhre K., Varagnol M., Claverie J.-M. J. Struct. Funct. Genomics 4:141-157(2003)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: May function as oxidoreductase.

Involvement in disease:

Subcellular Location:

Protein Families: Aldo/keto reductase family, Aldo/keto reductase 2 subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P76187

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A