Recombinant Escherichia coli Ornithine carbamoyltransferase subunit F (argF) | CSB-RP129074Ba
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Recombinant Escherichia coli Ornithine carbamoyltransferase subunit F (argF) | CSB-RP129074Ba | Cusabio
Alternative Name(s): OTCase-2
Gene Names: argF
Research Areas: Others
Organism: Escherichia coli (strain K12)
AA Sequence: SDLYKKHFLKLLDFTPAQFTSLLTLAAQLKADKKNGKEVQKLTGKNIALIFEKDSTRTRCSFEVAAFDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGHGQEVVETLAQYAGVPVWNGLTNEFHPTQLLADLMTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALTGLDLRLLAPKACWPEESLVAECSALAEKHGGKITLTEDVAAGVKGADFIYTDVWVSMGEAKEKWAERIALLRGYQVNAQMMALTDNPNVKFLHCLPAFHDDQTTLGKQMAKEFDLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMMATLGE
Tag Info: N-terminal 6xHis-tagged
Expression Region: 2-334aa
Sequence Info: Full Length of Mature Protein
MW: 40.7 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.
Reference: Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferases -- complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB.van Vliet F., Cunin R., Jacobs A., Piette J., Gigot D., Lauwereys M., Pierard A., Glansdorff N.Nucleic Acids Res. 12:6277-6289(1984)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.
Involvement in disease:
Subcellular Location: Cytoplasm
Protein Families: ATCase/OTCase family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P06960
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A